Verbascum nigrum : Cytotoxicity Evaluation in A431 Epidermoid Carcinoma Cells and Untargeted LC‐HR‐MS/MS Metabolite Profiling
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چکیده
منابع مشابه
Characterization and purification of a lipoxygenase inhibitor in human epidermoid carcinoma A431 cells.
A lipoxygenase inhibitor in the cytosolic fraction of human epidermoid carcinoma A431 cells was characterized and purified. The cytosolic inhibitor lost the inhibitory activity upon heating at 75 degrees C for 15 min or pretreating with 1 mg/ml trypsin at 37 degrees C for 60 min. Cytosol, after dialysis, lost the inhibitory activity but its inhibitory activity recovered when 1 mM GSH was added ...
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E-64-d (ethyl (2S, 3S)-3-[(S)-3-methyl-1-(3-methylbutylcarbamoyl) butylcarbamoyl]oxirane-2-carboxylate), a membrane-permeant derivative of the thiol protease-specific inhibitor E-64, was found to arrest human epidermoid carcinoma A431 cells at mitotic metaphase. This effect was dose-dependent with a threshold of 20 micrograms/ml in chemically defined culture medium. Cell cycle analysis by flow ...
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ژورنال
عنوان ژورنال: Chemistry & Biodiversity
سال: 2020
ISSN: 1612-1872,1612-1880
DOI: 10.1002/cbdv.202000644